Compatible with chemiluminescent substrates and fluorescent secondary antibodies. ![]() Western blotting: detection of the two unstained bands via the detection method used for the target protein.Western blot confirmation-detect the two unstained proteins (30 kDa and 80 kDa) using the detection system for the target protein.Fluorescent visualization-detect the 10 stained proteins using the 670 nm red laser or 700 nm channel of a fluorescence imager Unstained Protein Ladders MagicMark XP Western Protein Standard: 20220: 9: all SDS-PAGE gels: stain of choice : IgG binding sites: PageRuler Unstained Protein Ladder: 10200: 14: all SDS-PAGE gels: stain of choice : Strep-tag II sequence : PageRuler Unstained Broad Range Protein Ladder: 5250: 11: all SDS-PAGE gels: stain of choice.Method: Western blot analysis of EGFR was performed by loading serially diluted A431 cell lysate onto a NuPAGE 38 Tris-acetate gel (Cat. The protein ladder is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce, or add sample buffer prior to use.Ĭompare and view all other protein standards and ladders › Western blotting analysis of EGFR expression in A431 lysates transferred from an Novex 420 Tris-glycine gel and a NuPAGE 38 Tris-acetate gel using the iBlot 2 Gel Transfer Device. ![]() Recommended volume per well for 1.0 mm mini gelġ0 μL for electrophoresis, 5 μL for blottingĥ μL for electrophoresis, 2.IBright Prestained Protein Ladder contains twelve recombinant proteins, ten (11 to 250 kDa) that are blue-stained and fluor-labeled for direct and near-IR fluorescent visualization and protein sizing, and two (30 kDa and 80 kDa) that are unstained and contain IgG binding sites to bind the primary and secondary antibodies used for chemiluminescent and fluorescent detection of the target protein. ~160, 120, 80, 60, 50, 40, 30, 20, 15, 10 kDaĮach protein standard contains a 6X His-tag enabling the detection with the InVision His-tag In-gel StainĬolorimetric or methods that detect phosphorylated proteins such as Pro-Q Diamond phosphoprotein gel stainsĬolorimetric or methods that detect glycosylated proteins such as Pro-Q Glycoprotein stain kits Peppermint Stick Phosphoprotein Molecular Weight StandardsĬand圜ane Glycoprotein Molecular Weight Standards Tagged- proteins in the 10 -250 kDa range contain a Strep-tag™ II sequence and can be detected on western blots using Strep-Tactin™ conjugates or an antibody against Strep-tag™ II sequenceĭirect visualization can be achieved through standard staining protocols (e.g., Coomassie, etc.) The traditional method to visualize protein ladders on Western blots is to use prestained protein ladders which remain visible when transferred to the blotted membrane. Tagged-proteins contain an integral Strep-tag™ II sequence and can be detected on western blots using Strep-Tactin™ conjugates or an antibody against Strep-tag™ II sequence The ladder is conveniently packaged and ready to use with no heating, diluting or additional reducing agent necessary. For easy reference, the 100kDa, 50kDa and 20kDa protein bands have greater intensity than the other proteins (see website for product images). PageRuler Unstained Broad Range Protein LadderĪccurate estimation across a broader range stains or detected in Western blots with protein stains. Recommended Volume per well for 1.0 mm gel (μL)Ĭolorimetric, NIR fluorescence (700nm channel, blue bands), RGB fluorescence (550 nm channel, orange bands) ![]() Visible monitoring of gel separation and transfer efficiency Spectra Multicolor Broad Range Protein Ladderīest multicolor prestained ladder for routine applicationsĤ colors for improved visualization during separation and transferĪnalysis of high molecular weight proteins
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